Selected Publications

C.L. Hansen and F. Pelegri. Primordial germ cell specification in vertebrate embryos: Phylogenetic distribution and conserved molecular features of preformation and induction. (2021). Frontiers in Cell and Developmental Biology. doi: 10.3389/fcell.2021.730332

C.E. Moravec, G.C. Voit, J. Otterlee, and F. Pelegri. Identification of maternal-effect genes in zebrafish using maternal crispants. (2021). Development. doi: 10.1242/dev.199536
> preprint available at BioRxiv: doi: 10.1101/2021.02.19.432057

*S. Nair, *E.L. Welch, C.E. Moravec, R.L. Trevena, and F. Pelegri. A midbody component homolog, too much information/prc1-like, is required for microtubule reorganization during both cytokinesis and axis induction in the early zebrafish embryo. (preprint; 2021). BioRxiv. doi: 10.1101/2021.06.10.447958
*these authors contributed equally

C.L. Hansen and F. Pelegri. Methods for visualization of RNA and cytoskeletal elements in the early zebrafish embryo. (2021). Methods in Molecular Biology. doi:10.1007/978-1-0716-0970-5

C.E. Moravec and F. Pelegri. The role of the cytoskeleton in germ plasm aggregation and compaction in the zebrafish embryo. (2020). Current Topics in Developmental Biology. doi: 10.1016/bs.ctdb.2020.02.001.

*C. Eno , *C.L. Hansen, and F. Pelegri. Aggregation, segregation, and dispersal of homotypic germ plasm RNPs in the early zebrafish embryo. (2019). Developmental Dynamics. doi: 10.1002/dvdy.18.
*these authors contributed equally

C.E. Moravec and F. Pelegri. An accessible protocol for the generation of CRISPR-Cas9 knockouts using INDELs in zebrafish. (2019). Methods in Molecular Biology. doi: 10.1007/978-1-4939-9009-2_23.

C. Eno and F. Pelegri. Modulation of F-actin dynamics by maternal Mid1ip1L controls germ plasm aggregation and furrow recruitment in the zebrafish embryo. (2018). Development. doi: 10.1242/dev.156596.

C. Eno, T. Gomez, D.C. Slusarski, and F. Pelegri. Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo. (2018). Development. doi: 10.1242/dev.156604.

E.L. Welch, C. Eno, S. Nair, R.E. Lindeman, and F. Pelegri. Functional manipulation of maternal gene products using in vitro oocyte maturation in zebrafish. (2017). J Vis Exp. doi: 10.3791/55213

A. Hasley, S. Chavez, M. Danilchik, M. Wuhr, and F. Pelegri. Vertebrate embryonic cleavage pattern determination. (2017). Adv Exp Med Biol. 953:117-171. Review.

D.L. Baars, K.A. Takle, J. Heier, and F. Pelegri. Ploidy manipulation of zebrafish embryos with heat shock 2 treatment. (2016). J Vis Exp. doi:10.3791/54492.

C. Eno, B. Solanki, and F. Pelegri. aura/midipL1 regulates the cytoskeleton at the zebrafish egg-to-embryo transition. (2016) Development. 143(9):1585-99.

C. Eno and F. Pelegri. Germ cell determinant transmission, segregation, and function in the zebrafish embryo, Insights from Animal Reproduction. (2016) Dr. Rita Payan Carreira (Ed.), InTech, Chapter 5.

E. Welch and F. Pelegri. Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo. (2015) Bioarchitecture 5, 13-26.

J. Heier, K.A. Takle, A.O. Hasley, and F. Pelegri. Ploidy manipulation and induction of alternate cleavage patterns through inhibition of centrosome duplication in the early zebrafish embryo. (2015) Dev. Dyn. 244, 1300-1312.

X. Ge, D. Grotjahn, E. Welch,  C. Holguin, J. Lyman-Gingerich, E. Dimitrova, E. Abrams, F. Marlow, T. Yabe, M. Mullins, and F. Pelegri. Hecate/Grip2a acts to reorganize the cytoskeleton in the symmetry-breaking event of embryonic axis induction. (2014) PLoS Genetics 10, e1004422.

C. Eno and F. Pelegri. Gradual recruitment and selective clearing generate germ plasm aggregates in the zebrafish embryo. (2013) Bioarchitecture 3, 125-132.

S. Nair, F. Marlow, E. Abrams, L. Kapp, M. Mullins, and F. Pelegri. The chromosomal passenger protein Birc5b organizes microfilaments and germ plasm in the zebrafish embryo. (2013) PLoS Genetics 9, e1003448.

S. Nair, R.E. Lindeman, and F. Pelegri.  In vitro oocyte culture-based manipulation of zebrafish maternal genes. (2013) Dev Dyn. 242(1):44-52

R.E. Lindeman and F. Pelegri. Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote. (2012) Current Biology 22, 843-851.

F. Pelegri and M.C. Mullins. Genetic screens for mutations affecting adult traits and parental-effect genes. (2011) Methods Cell Biol. 104:83-120

S. Nair and F.J. Pelegri. Practical approaches for implementing forward genetic strategies in zebrafish. (2011) Methods Mol Biol. 770:185-209.

E. Putiri and F. Pelegri. The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes. (2011) Dev. Biol. 353, 275-289.

R.E. Lindeman and F. Pelegri. Vertebrate maternal-effect genes: Insights into fertilization, early cleavage divisions, and germ cell determinant localization from studies in the zebrafish. (2010) Mol Reprod Dev. 77(4):299-313.

T. Yabe, X. Ge, R. Lindeman, S. Nair, G. Runke, M.C. Mullins, and F. Pelegri. The maternal-effect gene cellular island encodes Aurora B kinase and is essential for furrow formation in the early zebrafish embryo. (2009) PloS Genetics 5, e1000518.

T. Yabe, X. Ge, and F. Pelegri. The zebrafish maternal-effect gene cellular atoli encodes the centriolar component Sas-6 and defects in its paternal function promote whole genome duplication. (2007). Developmental Biology, 312:44-60.

D.C. Slusarski and F. Pelegri. Calcium signaling in vertebrate embryonic patterning and morphogenesis. (2007) Dev Biol. 307(1):1-13.

L. Urven, T. Yabe, and F. Pelegri. A role for non-muscle myosin II function in furrow maturation in the early zebrafish embryo. (2006) Journal of Cell Science. 119:4342-4352.

J. Lyman-Gingerich, R. Lindeman, E. Putiri, K. Stolzmann, and F. Pelegri. The analysis of axis induction mutant embryos reveals morphogenetic movements associated with zebrafish yolk extension. (2006) Developmental Dynamics. 235:2749-2760.

E.V. Theusch, K.J. Brown, and F. Pelegri. Separate pathways of RNA recruitment lead to the compartmentalization of the zebrafish germ plasm. (2006) Developmental Biology. 292:129-141.

J. Lyman Gingerich, T.A. Westfall, D.C. Slusarski, and F. Pelegri. hecate, a zebrafish maternal effect gene, affects dorsal organizer induction and intracellular calcium transient frequency. (2005) Dev Biol. 286(2):427-39.

F. Pelegri and M.C. Mullins. Genetic screens for maternal-effect mutations. (2004) Methods Cell Biol. 77:21-51.

F. Pelegri, M.P. Dekens, S. Schulte-Merker, H.M. Maischein, C. Weiler, and C. Nusslein-Volhard. Identification of recessive maternal-effect mutations in the zebrafish using a gynogenesis-based method. (2004) Dev Dyn. 231(2):324-35.

F. Pelegri. Maternal factors in zebrafish development. (2003) Dev Dyn. 228(3):535-54.