Recent Publications

Hansen CL, Pelegri F. Methods for visualization of RNA and cytoskeletal elements in the early zebrafish embryo. (in press). Methods in Molecular Biology. doi:10.1007/978-1-0716-0970-5

Moravec CE, Pelegri F. The role of the cytoskeleton in germ plasm aggregation and compaction in the zebrafish embryo. (2020). Current Topics in Developmental Biology. doi: 10.1016/bs.ctdb.2020.02.001.

*Eno C, *Hansen CL, Pelegri F. Aggregation, segregation, and dispersal of homotypic germ plasm RNPs in the early zebrafish embryo. (2019). Developmental Dynamics. doi: 10.1002/dvdy.18.
*these authors contributed equally

Moravec CE, Pelegri F. An accessible protocol for the generation of CRISPR-Cas9 knockouts using INDELs in zebrafish. (2019). Methods in Molecular Biology. doi: 10.1007/978-1-4939-9009-2_23.

Eno C, Pelegri F. Modulation of F-actin dynamics by maternal Mid1ip1L controls germ plasm aggregation and furrow recruitment in the zebrafish embryo. (2018). Development. doi: 10.1242/dev.156596.

Eno C, Gomez T, Slusarski DC, Pelegri F. Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo. (2018). Development. doi: 10.1242/dev.156604.

Welch EL, Eno CC, Nair S, Lindeman RE, Pelegri F. Functional manipulation of maternal gene products using in vitro oocyte maturation in zebrafish. (2017). J Vis Exp. doi: 10.3791/55213

Hasley A, Chavez S, Danilchik M, Wuhr M, Pelegri F. Vertebrate embryonic cleavage pattern determination. (2017). Adv Exp Med Biol. 953:117-171. Review.

Baars DL, Takle KA, Heier J, Pelegri F. Ploidy manipulation of zebrafish embryos with heat shock 2 treatment. (2016). J Vis Exp. doi:10.3791/54492.

Zampedri, C., Tinoco-Cuellar, M., Carrilo-Rosas, S., Diaz-Tellez, A., Ramos-Balderas, J.L., Pelegri, F., Maldonado, E. P54 RNA helicases are cytoplasmic granules residents that are required for development and stress resilience. (2016) Biol Open. 015826

Eno, C., Solanki, B., Pelegri, F. aura/midipL1 regulates the cytoskeleton at the zebrafish egg-to-embryo transition. (2016) Development. 143(9):1585-99.

Eno, C., Pelegri, F. Germ cell determinant transmission, segregation, and function in the zebrafish embryo, Insights from Animal Reproduction. (2016) Dr. Rita Payan Carreira (Ed.), InTech, Chapter 5.

Welch, E., Pelegri, F. Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo. (2015) Bioarchitecture 5, 13-26.

Heier, J., Takle, K.A., Hasley, A.O., Pelegri, F. Ploidy manipulation and induction of alternate cleavage patterns through inhibition of centrosome duplication in the early zebrafish embryo. (2015) Dev. Dyn. 244, 1300-1312.

Ge, X., Grotjahn, D., Welch, E., Holguin, C., Lyman-Gingerich, J., Dimitrova, E., Abrams, E., Marlow, F., Yabe, T., Mullins, M., Pelegri, F. Hecate/Grip2a acts to reorganize the cytoskeleton in the symmetry-breaking event of embryonic axis induction. (2014) PLoS Genetics 10, e1004422.

Eno, C., Pelegri, F. Gradual recruitment and selective clearing generate germ plasm aggregates in the zebrafish embryo. (2013) Bioarchitecture 3, 125-132.

Nair, S., Marlow, F., Abrams, E., Kapp, L., Mullins, M., Pelegri, F. The chromosomal passenger protein Birc5b organizes microfilaments and germ plasm in the zebrafish embryo. (2013) PLoS Genetics 9, e1003448.

Nair, S., Lindeman, R.E., and Pelegri F.  In vitro oocyte culture-based manipulation of zebrafish maternal genes. (2013) Dev Dyn. 242(1):44-52

Lindeman, R.E., Pelegri, F. Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote. (2012) Current Biology 22, 843-851.

Pelegri, F., Mullins, M.C. Genetic screens for mutations affecting adult traits and parental-effect genes. (2011) Methods Cell Biol. 104:83-120

Nair, S., Pelegri, F.J. Practical approaches for implementing forward genetic strategies in zebrafish. (2011) Methods Mol Biol. 770:185-209.

Putiri, E. and Pelegri, F. The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes. (2011) Dev. Biol. 353, 275-289.

Branam, A.M., Hoffman, G.G., Pelegri, F., Greenspan, D.S. Zebrafish chordin-like and chordin are functionally redundant in regulating patterning of the dorsoventral axis. (2010) Dev Biol. 15;341(2):444-58.

Hoffman, G.G., Branam, A.M., Huang, G., Pelegri, F., Cole, W.G., Wenstrup, R.M., Greenspan, D.S. Characterization of the six zebrafish clade B fibrillar procollagen genes, with evidence for evolutionarily conserved alternative splicing within the pro-alpha1(V) C-propeptide. (2010) Matrix Biol. 29(4):261-75.

Lindeman, R.E., Pelegri, F. Vertebrate maternal-effect genes: Insights into fertilization, early cleavage divisions, and germ cell determinant localization from studies in the zebrafish. (2010) Mol Reprod Dev. 77(4):299-313.

Kim, Y.C., Clark, R.J., Pelegri, F., Alexander, C.M. Wnt4 is not sufficient to induce lobuloalveolar mammary development. (2009) BMC Dev Biol. 9:55.

Yabe, T., Ge. X., Lindeman R., Nair, S., Runke G., Mullins M.C., and Pelegri, F. The maternal-effect gene cellular island encodes Aurora B kinase and is essential for furrow formation in the early zebrafish embryo. (2009) PloS Genetics 5, e1000518.

Yabe, T., Ge, X. and F. Pelegri. The zebrafish maternal-effect gene cellular atoli encodes the centriolar component Sas-6 and defects in its paternal function promote whole genome duplication. (2007). Developmental Biology, 312:44-60.

Slusarski, D.C., Pelegri, F. Calcium signaling in vertebrate embryonic patterning and morphogenesis. (2007) Dev Biol. 307(1):1-13.

Jasuja, R., Ge, G., Voss, N.G., Lyman-Gingerich, J., Branam, A.M., Pelegri, F.J., Greenspan, D.S. Bone morphogenetic protein 1 prodomain specifically binds and regulates signaling by bone morphogenetic proteins 2 and 4. (2007) J Biol Chem. 282(12):9053-62.

Urven, L., Yabe, T. and F. Pelegri. A role for non-muscle myosin II function in furrow maturation in the early zebrafish embryo. (2006) Journal of Cell Science. 119:4342-4352.

Lyman-Gingerich, J., Lindeman, R., Putiri, E., Stolzmann, K. and Pelegri, F. The analysis of axis induction mutant embryos reveals morphogenetic movements associated with zebrafish yolk extension. (2006) Developmental Dynamics. 235:2749-2760.

Jasuja R., Voss, N., Ge, G., Hoffman, G.G., Lyman-Gingerich, J., Pelegri, F., Greenspan, D.S. bmp1 and mini fin are functionally redundant in regulating formation of the zebrafish dorsoventral axis. (2006). Mech Dev. 123(7):548-58.

Theusch, E.V., Brown, K.J. and Pelegri, F. Separate pathways of RNA recruitment lead to the compartmentalization of the zebrafish germ plasm. (2006) Developmental Biology. 292:129-141.

Lyman Gingerich, J., Westfall, T.A., Slusarski, D.C., Pelegri, F. hecate, a zebrafish maternal effect gene, affects dorsal organizer induction and intracellular calcium transient frequency. (2005) Dev Biol. 286(2):427-39.

Pelegri, F., Mullins, M.C. Genetic screens for maternal-effect mutations. (2004) Methods Cell Biol. 77:21-51.

Pelegri, F., Dekens, M.P., Schulte-Merker, S., Maischein, H.M., Weiler, C., Nusslein-Volhard, C. Identification of recessive maternal-effect mutations in the zebrafish using a gynogenesis-based method. (2004) Dev Dyn. 231(2):324-35.

Pelegri, F. Maternal factors in zebrafish development. (2003) Dev Dyn. 228(3):535-54.

Dekens, M.P., Pelegri, F.J., Maischen, H.M., Nusslein-Volhard, C. The maternal-effect gene futile cycle is essential for pronuclear congression and mitotic spindle assembly in the zebrafish zygote. (2003) Development. 130(17):3907-16.